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human liver total rna  (TaKaRa)


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    TaKaRa human liver total rna
    Human Liver Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human liver total rna/product/TaKaRa
    Average 95 stars, based on 372 article reviews
    human liver total rna - by Bioz Stars, 2026-03
    95/100 stars

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    Quantification of L1PA subfamily RNA signals in human liver using subfamily-selective PCR primers. ( a ) LINE1 RT-qPCR analysis of human liver RNA using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 technical replicates. ( b ) RNA-seq analysis of L1PA subfamilies in human liver using publicly available data. Summed FPKM values of annotated loci per L1PA subfamily are shown. Data are presented as mean ± s.d., n = 3 biological replicates. Data include multi-mapping reads; a corresponding analysis restricted to unique alignments is presented in Supplementary Fig. 3. ( c ) LINE1 RT-qPCR analysis of HEK293T cells following treatment with 5-azacytidine using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 biological replicates. Statistical significance was assessed using a two-tailed t-test and *, ** and *** indicate p < 0.05, 0.01 and 0.001, respectively.

    Journal: Scientific Reports

    Article Title: Subfamily-selective PCR primers for the human LINE1 L1PA lineage

    doi: 10.1038/s41598-025-17649-z

    Figure Lengend Snippet: Quantification of L1PA subfamily RNA signals in human liver using subfamily-selective PCR primers. ( a ) LINE1 RT-qPCR analysis of human liver RNA using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 technical replicates. ( b ) RNA-seq analysis of L1PA subfamilies in human liver using publicly available data. Summed FPKM values of annotated loci per L1PA subfamily are shown. Data are presented as mean ± s.d., n = 3 biological replicates. Data include multi-mapping reads; a corresponding analysis restricted to unique alignments is presented in Supplementary Fig. 3. ( c ) LINE1 RT-qPCR analysis of HEK293T cells following treatment with 5-azacytidine using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 biological replicates. Statistical significance was assessed using a two-tailed t-test and *, ** and *** indicate p < 0.05, 0.01 and 0.001, respectively.

    Article Snippet: cDNA synthesis was performed on human liver RNA (Amsbio R1234151-50) with SuperScript II Reverse Transcriptase (ThermoFisher).

    Techniques: Quantitative RT-PCR, Standard Deviation, RNA Sequencing, Two Tailed Test